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14 DPO: Your Test Result and What Comes Next

The end of the two-week wait, and how to read whatever you find.

Day 14 of the two-week wait, drawn as a growing plant

Quick answer

At 14 DPO, median urine hCG was about 137 mIU/mL in a daily study of women who conceived, so most pregnancies show on any home test. Still, an NIH study found 10% of pregnancies were undetectable on the first day of a missed period. If your test is negative and your period is late, retest in a few days.

Key takeaways

  • Median urine hCG at 14 DPO was 137.19 mIU/mL; 90% of pregnancies were above 45 mIU/mL.
  • The FDA says 10 to 20 of every 100 pregnant women will not get a positive on the first day of a missed period.
  • The highest possible detection rate was estimated at 90% on the first missed day and 97% a week later.
  • Negative with no period: retest in a few days, then contact a clinician if it stays negative and your period does not come.

Fourteen days past ovulation is the end of the classic two-week wait. For many people, it is the day the period is due or already late. A home test now gives a clear answer for most pregnancies, but not quite all of them. This page explains how much to trust today's result, what to do after a negative or a positive, and when it is time to talk to a doctor.

What happens at 14 DPO

If you are pregnant, hCG has been rising for several days and keeps the corpus luteum going, which holds off your period.10 If you are not, the corpus luteum breaks down about 14 days after ovulation and your period follows.10 Fourteen days is a textbook figure, though: in more than 600,000 app-tracked cycles, the average luteal phase was 12.4 days, and most fell between 7 and 17 days.11 A period at 12 or 16 DPO can be normal for you.

14 DPO symptoms

Missed or late period aside, symptoms at 14 DPO still overlap heavily with premenstrual ones. Some people begin to notice early pregnancy signs around now, while many do not for weeks. A test is the better guide today.

Can you get a positive pregnancy test at 14 DPO?

Yes, in the large majority of pregnancies. In daily urine samples from 109 women who conceived, median hCG at 14 DPO was 137.19 mIU/mL, and 90% of pregnancies were above 45.06 mIU/mL.1 That clears the threshold of nearly every home test. In a 2011 lab comparison, First Response tests detected 97% of 120 pregnancies on the day of the missed period.5 First Response says its Early Result test is more than 99% accurate from the day of the missed period.4

Yet some pregnancies are not detectable on this day at all. An NIH study of 136 clinical pregnancies found that 10% had not yet implanted by the first day of the missed period, so the highest possible detection rate for any hCG test was 90% that day, rising to 97% one week later.3 The FDA puts it plainly: 10 to 20 of every 100 pregnant women will not detect their pregnancy on the first day of a missed period.2

14 DPO BFN: what it means and what to do

A negative at 14 DPO makes pregnancy much less likely if your ovulation date is right. If it was a prediction rather than a confirmed date, you may simply be earlier than you think. Here is a calm plan:

WhenWhat to do
Today, negative, no periodNote the result. Until you are sure, the FDA suggests avoiding alcohol, smoking and medicines you don't need2
2 to 3 days later, still no periodRetest with first-morning urine, which usually has more hCG2
About 7 days after a negative, still no periodRetest; First Response advises calling your doctor if the second test is also negative4
Period startsThis cycle is over. See below for support and next steps

14 DPO and implantation

The implantation window has closed for continuing pregnancies, which in the NIH study implanted between 6 and 12 days after ovulation.6 Spotting now is most often the start of a period. If you had a positive test and then bleeding, it may have been an early loss: in the same study, 25% of detected conceptions ended before 6 weeks after the last period.6 Our chemical pregnancy page explains this gently. For a recap of spotting earlier in the wait, see our implantation bleeding guide.

What to do now

If you are positive, contact your clinician or midwife to arrange care, and keep taking 400 mcg of folic acid a day.9 Our first prenatal appointment guide explains what to expect.

After a positive test, get same-day care for one-sided pelvic pain or abnormal bleeding. Call 911 or go to the ER for severe, sudden lower belly pain, shoulder pain, or fainting, which can be signs of a ruptured ectopic pregnancy.8

If you are negative, follow the plan above. Use the calculator to double-check your dates for next cycle:

DPO calculator

How many days past ovulation are you, and when can a pregnancy test tell? Enter your ovulation day, or your last period if you don’t know it.

If your period has started, give yourself time. Our page on coping with the two-week wait has support for this moment too. The CDC suggests seeing a health care provider after a year of trying under 35, after 6 months at 35 or older, and sooner over 40 or with irregular periods or more than one miscarriage.7 Our guide on when to see a fertility specialist goes further. Not pregnant this cycle? Track smarter next time, starting from day 1 of your next cycle, and come back to 1 DPO when you ovulate.

Frequently asked questions

Yes, but it becomes less likely the later you test. In daily urine data, 90% of pregnancies had more than 45 mIU/mL of hCG at 14 DPO. The most common cause of a false negative now is ovulating later than you counted. If your period has not started, retest in two to three days.

Very accurate when positive, and fairly reliable when negative if your ovulation date is right. First Response says its Early Result test is more than 99% accurate from the day of the missed period. An NIH study estimated that even a perfect hCG test could detect at most 90% of pregnancies on the first missed day, rising to 97% a week later.

Often, it means ovulation happened later than you think, so you are earlier in the wait than your count says. Your period may also be about to start. Retest in a few days. If you get a second negative and your period still has not come, the First Response instructions advise calling your doctor.

Contact your clinician or midwife to arrange care, and keep taking 400 mcg of folic acid daily. Seek care the same day for one-sided pelvic pain or abnormal bleeding, and emergency care for severe sudden pain, shoulder pain or fainting, which can be signs of an ectopic pregnancy.

The CDC suggests seeing a health care provider after one year of trying if you are under 35, after 6 months if you are 35 or older, and sooner if you are over 40 or have warning signs such as irregular or absent periods or more than one miscarriage.

This page is educational and is not medical advice. Talk to a clinician about your own situation, and seek urgent care for heavy bleeding, severe pain or fainting.

Keep reading

Next step · 4 of 6

Investigate

Test both partners. At-home tests are a start; a specialist visit is the answer.

Sources

  1. 1Strips of Hope: Accuracy of Home Pregnancy Tests and New Developments. Geburtshilfe und Frauenheilkunde. Gnoth C, Johnson S, 2014. Table 2: daily urine hCG by day since ovulation in 109 UK women. Johnson is employed by the maker of Clearblue tests
  2. 2Pregnancy (home use tests). U.S. Food and Drug Administration. FDA consumer guidance on home pregnancy tests
  3. 3Natural limits of pregnancy testing in relation to the expected menstrual period. JAMA. Wilcox AJ, Baird DD, Dunson D, McChesney R, Weinberg CR, 2001. 136 clinical pregnancies
  4. 4First Response Early Result Pregnancy Test. First Response. Brand product page and FAQ, checked October 2026
  5. 5The utility of six over-the-counter (home) pregnancy tests. Clinical Chemistry and Laboratory Medicine. Cole LA, 2011. Six home tests; 120 pregnancies on the day of missed menses
  6. 6Time of implantation of the conceptus and loss of pregnancy. New England Journal of Medicine. Wilcox AJ, Baird DD, Weinberg CR, 1999. 189 conceptions analyzed, 48 early losses
  7. 7Infertility FAQs. Centers for Disease Control and Prevention. When to see a health care provider
  8. 8Ectopic pregnancy. MedlinePlus (U.S. National Library of Medicine). Reviewed March 2026
  9. 9About Folic Acid. Centers for Disease Control and Prevention. 400 mcg daily
  10. 10Menstrual Cycle. MSD Manual Consumer Version. McLaughlin JE, reviewed May 2025. Corpus luteum and hCG
  11. 11Real-world menstrual cycle characteristics of more than 600,000 menstrual cycles. npj Digital Medicine. Bull JR et al., 2019. 612,613 cycles; funded by Natural Cycles

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